Buffer Calculator (Beta)

Use this free online buffer calculator to prepare common laboratory buffers by target pH, strength, salt or hydrate form, and final volume, with pH-suitability guidance and fixed recipes for PBS, TBS, TAE, TBE, SSC, and TE.

Buffer calculator inputs

Type to search or click the field to choose from the buffer list.

References

References for buffer calculator logic: Henderson-Hasselbalch buffer pH ratios, pKa-guided buffer ranges, Good's biological buffers, enzyme-assay buffer preparation, and salt or hydrate molecular-weight checks. Mol Biology Tools implementation code is original.

  1. Henderson-Hasselbalch acid/base ratio logic: Henderson (1908), acid strength and buffering capacity.
  2. Biological buffer pH ranges: Good et al. (1966), hydrogen ion buffers for biological research.
  3. Enzyme-assay buffer preparation context: Gomori (1955), preparation of buffers for enzyme studies.
  4. Salt, hydrate, and acid/base molecular-weight checks: PubChem Compound database.