Use this to dilute a stock solution to a desired final concentration and volume using C1V1 = C2V2.
Useful Checks
If the stock volume is too small to pipette directly, the result can flag the problem and point you toward an intermediate dilution.
Use this with the Molarity Calculator when you first prepare a stock solution and then need a working dilution.
The calculator handles normal C1V1 dilution and compatible unit conversions in the same workflow.
It can convert between molar units and mass or percent units when molecular weight is known, and asks for molecular weight only when that conversion needs it.
The result keeps stock volume, diluent volume, and final volume separate, with practical display units for small and large volumes.
Minimum Inputs Needed
Stock concentration.
Desired final concentration.
Desired final volume.
Optional Settings
Molecular weight required
Only needed when converting between molar units and mass or % w/v units.
Available stock volume (optional)
Checks whether you have enough stock for the requested dilution.
Stock label (optional)
Adds a name to the result, useful when working with many stocks.
Concentration and volume units
Choose units that match your stock and final solution.
How To Use
Enter the stock concentration.
Enter the desired final concentration.
Enter the final volume.
Add molecular weight only if the tool asks for it.
Click Calculate dilution.
Understanding The Results
The quick result shows how much stock to take.
It also shows how much diluent to add.
The final volume is the total volume after stock plus diluent.
If the stock volume is very small, the tool may suggest an intermediate dilution.
If available stock is entered, the tool warns if you do not have enough.
Accepted Input Formats
Concentration units include M, mM, µM, nM, mg/mL, µg/mL, ng/µL, ng/mL, X, and % w/v.
Volume units include L, mL, µL, and nL.
X dilutions work with X units only.
Assumptions And Limitations
The calculation assumes ideal dilution.
It does not account for density or volume contraction after mixing.
Very small volumes should be handled with appropriate pipettes or intermediate dilution.
Example
To dilute 10 mM stock to 1 mM in 100 µL final volume, enter 10 mM, 1 mM, and 100 µL. The result is 10 µL stock plus 90 µL diluent.
Use note: These tools are for research and educational planning. Check important calculations and sequence designs before ordering reagents or running experiments.