DNA Ligation Calculator

Calculate the insert DNA mass required for a vector-insert ligation with this free online DNA ligation calculator, using vector mass, vector and insert lengths, and the target insert-to-vector molar ratio.

Ligation calculator inputs

References

References for DNA ligation calculator logic: insert-to-vector molar ratio, double-stranded DNA molecular-weight approximation, fmol estimates, and T4 DNA ligase workflow context. Mol Biology Tools implementation code is original.

  1. Insert-to-vector molar-ratio formula: New England Biolabs NEBioCalculator.
  2. Double-stranded DNA molecular-weight approximation: Thermo Fisher DNA and RNA molecular weights and conversions.
  3. Ligation workflow context: New England Biolabs T4 DNA Ligase product and protocol information.