DNA Ligation Calculator

Calculate the insert DNA mass required for a vector-insert ligation with this free online DNA ligation calculator, using vector mass, vector and insert lengths, and the target insert-to-vector molar ratio.

Ligation calculator inputs

References

Sources for insert-to-vector ratios, dsDNA mass-to-mole estimates, and ligation workflow context. Calculations use original Mol Biology Tools code and the 660 g/mol per base-pair approximation.

  1. Insert-to-vector molar-ratio formula: New England Biolabs NEBioCalculator.
  2. Double-stranded DNA molecular-weight approximation: IDT DNA mass and copy-number conversion guide (2024). Uses 660 g/mol per dsDNA base pair; fmol values are approximate, not sequence-specific molecular weights.
  3. Ligation workflow context: New England Biolabs T4 DNA Ligase product and protocol information.