Site-Directed Mutagenesis Primer Designer (Beta)
Design primers for single or multiple nucleotide substitutions in one final sequence.
Your mutagenesis design
PCR plan
Primers 5′ → 3′
Blue = added or rewritten 5′ region; dark = exact template-binding region. Tm covers the exact 3′ binding region.
| Primer / reaction | Sequence (5′ → 3′) | Length | Binding Tm | PCR product |
|---|
Overlaps
Edited sequence
Coordinates retain the original sequence origin. GenBank exports mark the substitutions; imported gene features are not transferred.