ORF & Protein Translator

Use this free online ORF finder and DNA to protein translator to identify open reading frames, inspect coding regions, translate DNA into amino acid sequences, and reverse-translate protein input in Quick translate.

ORF and protein translator inputs
Tool mode
No sequence loaded.

Accepted file types: .txt, .fa, .fasta, .fna, .ffn, .seq, .gb, .gbk, .genbank. Plain-text DNA, FASTA, or GenBank text only.

Circular DNA/plasmids are not handled as circular yet. For regions that cross base 1, paste the relevant region as linear FASTA or raw DNA sequence.

The frame view filters translation, ATG start markers, frame summary, ORF map, ORF table, and copied ORF outputs.

References

References for ORF finder and DNA-to-protein translation logic: NCBI genetic code tables, alternative translation codes, ambiguous DNA handling, and FASTA input parsing. Mol Biology Tools implementation code is original.

  1. Genetic-code translation tables: NCBI Genetic Codes table.
  2. Ambiguous DNA handling: NC-IUB/IUPAC nucleotide ambiguity recommendations.
  3. FASTA input parsing: NCBI FASTA format documentation.