Use this to translate DNA, inspect reading frames, and find possible ORFs. The quick mode handles fast DNA-to-protein or protein-to-DNA checks; expression still needs biology, not just a long ORF.
Useful Checks
Use the checked coding region with Primer Designer when you need PCR primers around an ORF or insert.
Use ORF results with Codon Optimizer when moving from a DNA coding sequence to expression planning.
Minimum Inputs Needed
For ORF finder: DNA sequence.
For quick translate: DNA or protein sequence, depending on the selected input type.
Optional Settings
Mode
Choose ORF finder for full analysis. Choose Quick translate for a fast conversion.
Frame view
Analyze all six frames or only one selected frame.
Minimum ORF length, aa
Hides very short ORFs.
Max ORFs shown
Keeps large results readable.
ORF table order
Changes how ORFs are sorted in the table.
Protein line wrap
Controls how protein sequences are wrapped.
Search region
Analyze the full sequence or only a selected region.
Include open-ended ORFs
Also show ORFs that do not have a downstream stop codon in the selected sequence.
Quick DNA frame / output
Choose the frame and whether to show one frame or all six frames.
How To Use
Choose ORF finder or Quick translate.
Paste or upload the sequence.
Adjust frame, region, or ORF length only if needed.
Click Analyze sequence or use the quick result.
Understanding The Results
Frame summaries show translations and stop codons.
The ORF table shows position, strand, frame, length, and sequence actions.
Protein FASTA copies the translated protein.
ORF DNA FASTA gives the coding-strand DNA in 5'->3' direction.
Accepted Input Formats
DNA or FASTA for ORF finder.
DNA or protein for quick translate.
FASTA headers are ignored.
Ambiguous codons may translate as X.
Assumptions And Limitations
An ORF is a sequence prediction, not proof of expression.
The tool uses standard start-to-stop ORF rules for routine checks.
Use biological context before deciding which ORF is real.
Example
Paste a coding sequence, keep all six frames selected, set a minimum ORF length, and copy the likely protein sequence from the ORF table.
Use note: These tools are for research and educational planning. Check important calculations and sequence designs before ordering reagents or running experiments.