Check where primers bind on a DNA template and review expected amplicons, orientation, and possible off-target sites.
Primer Binding Checker is a browser-based tool for checking whether one or multiple primer pairs bind the expected sites on linear and circular DNA.
Use note: Paste primers or receive designed PCR primers, then confirm orientation, mismatches, and expected amplicon size before ordering.
Accepted file types: .txt, .fa, .fasta, .fna, .ffn, .seq, .gb, .gbk, .genbank. Maximum parsed template length: 500,000 bp.
Transfer the current validated template sequence to another Mol Biology Tools page in a new tab.