Restriction Site Analyzer

Find restriction enzyme sites, cut positions, and restriction maps for DNA, FASTA, or GenBank sequences.

Restriction Site Analyzer is a browser-based tool for finding restriction enzyme recognition sites in linear or circular DNA during restriction digest planning.

Use note: Send validated sequences to Primer Designer or Primer Binding Checker for primer binding validation.

Accepted file types: .txt, .fa, .fasta, .fna, .ffn, .seq, .gb, .gbk, .genbank. Plain-text DNA, FASTA, or GenBank text only.

Use circular topology for plasmids if sites crossing base 1 or circular digest fragments matter.

Send sequence to another tool

Send the current sequence to another Mol Biology Tools page. The sequence will open in a new tab.