Reverse Complement Tool

Use this free online reverse complement tool to generate a reverse complement, complement, reversed sequence, or cleaned DNA from plain-text, FASTA, or GenBank input, with full IUPAC ambiguity-code support.

Reverse complement inputs
No sequence parsed yet.

Accepted file types: .txt, .fa, .fasta, .fna, .ffn, .seq, .gb, .gbk, .genbank. Plain-text DNA, FASTA, or GenBank only.

References

Sources for IUPAC DNA-base complements, FASTA input, and GenBank ORIGIN parsing. Sequence transformations use original Mol Biology Tools code.

  1. Ambiguous DNA-base complements: NC-IUB (1985), nucleotide ambiguity nomenclature. These symbols define the supported ambiguous bases and their complements.
  2. FASTA input parsing: NCBI FASTA format documentation.
  3. GenBank ORIGIN text parsing: NCBI GenBank sample record documentation.