Reverse Complement Tool

Use this free online reverse complement tool to generate a reverse complement, complement, reversed sequence, or cleaned DNA from plain-text, FASTA, or GenBank input, with full IUPAC ambiguity-code support.

Reverse complement inputs
No sequence parsed yet.

Accepted file types: .txt, .fa, .fasta, .fna, .ffn, .seq, .gb, .gbk, .genbank. Plain-text DNA, FASTA, or GenBank only.

References

References for reverse complement generation: IUPAC ambiguous DNA-base complements, FASTA sequence parsing, and GenBank ORIGIN text handling. Mol Biology Tools implementation code is original.

  1. Ambiguous DNA-base complements: NC-IUB/IUPAC nucleotide ambiguity recommendations.
  2. FASTA input parsing: NCBI FASTA format documentation.
  3. GenBank ORIGIN text parsing: NCBI GenBank sample record documentation.