Help Guides

Short guides for each tool: required inputs, settings worth changing, result checks, and useful handoffs.

Primer Designer

Design PCR primers, add cloning overhangs only when needed, and send the pair for binding checks.

Check one or multiple primer pairs against the same template and review product size.

Plan sequencing primers, reliable read coverage, mutation-priority checks, and transfer to Primer Binding Checker.

Find enzyme sites, zero/single cutters, map positions, and digest fragments.

Reverse-complement, complement, reverse, and clean DNA sequence.

Translate DNA, find ORFs, and copy protein or coding-DNA FASTA.

Optimize coding sequences, check translation, and send the optimized CDS to Primer Designer.

Calculate insert DNA amount for ligation setup.

Calculate single-solute masses and multi-solute buffer, media, stock, staining, and assay recipes.

Calculate stock and diluent volumes for a dilution.