Help Guides
Step-by-step help for every tool: inputs, settings, examples and results. Each guide also explains which tools it can send data to, what is transferred, why to use the next tool, and when to copy results manually.
Plasmid Editor (Beta)
Open annotated DNA, edit bases and features, review deletions, and save projects or export sequence files and SVG, PNG or JPEG maps.
PCR Primer Designer
Design PCR primers with a fixed binding length or target Tm, add optional cloning tails, and send the pair for a binding check.
Primer Binding Checker
Map exact forward and reverse primer binding sites, identify 5' tails and additional matches, and predict PCR product size.
Primer Scan (Beta)
Screen existing primers against a host or custom reference, review gene context where available, and flag host products close to an expected PCR size.
Sanger Primer Designer
Design up to 10 Sanger primer pairs, plan primer walking, assess read coverage, check mutation positions, and validate template binding.
Assembly PCR Primer Designer (Beta)
Design assembly PCR primers for NEBuilder HiFi, GeneArt, In-Fusion and overlap PCR. Set fragment order, overlaps and optional linkers.
Gibson Assembly Primer Designer (Beta)
Design Gibson assembly primers online. Set vector ends, order DNA fragments, edit overlaps and export primers and the assembled plasmid sequence.
Site-Directed Mutagenesis Primer Designer (Beta)
Choose Overlap PCR, Overlap assembly or Inverse PCR for nucleotide substitutions, then review the primers and reaction plan.
Restriction Site Analyzer
Use the restriction mapper to compare enzymes, predict digest fragments, and review linear or circular maps and a virtual gel.
Reverse Complement Tool
Generate reverse-complement, complement or reversed DNA from plain-text, FASTA or GenBank input, with IUPAC ambiguity codes.
ORF Finder and Protein Translator
Find open reading frames, translate DNA to protein, or reverse-translate a protein into possible or degenerate coding DNA.
Codon Optimizer
Optimize a coding sequence for a selected host while preserving the protein, then review codon changes, GC content, and primer options.
DNA Ligation Calculator
Calculate insert DNA mass for a vector-insert ligation from fragment lengths, vector mass, and the target insert-to-vector molar ratio.
Molarity Calculator
Calculate single-solute mass, molarity, final volume, and g/L; convert protein molarity with kDa; or build multi-solute recipes.
Buffer Calculator
Prepare named buffers by concentration, volume, and target pH, using acid/base calculations or fixed templates for PBS, TBS, TAE, TBE, SSC, and TE.
Dilution Calculator
Calculate C1V1 stock dilutions or antibody working solutions from 1:N ratios or stock-to-target concentrations, with small-volume guidance.