Help Guides

Step-by-step help for every tool, including required inputs, optional settings, result interpretation, limitations, examples, and related workflows.

PCR Primer Designer

Design PCR primer pairs for a target Tm, add cloning overhangs, review primer quality, and send selected pairs for binding checks.

Map forward and reverse primer binding sites, account for 5' overhangs, flag additional matches, and predict PCR product size.

Design Sanger sequencing primers, plan primer walking, assess read coverage, check mutation positions, and validate template binding.

Use the restriction mapper to compare enzymes, predict digest fragments, and review linear or circular maps and a virtual gel.

Generate reverse-complement, complement, or reversed DNA from plain-text or FASTA input, including IUPAC ambiguity codes.

Find open reading frames, translate DNA to protein, or reverse-translate a protein into possible or degenerate coding DNA.

Optimize a coding sequence for a selected host while preserving the protein, then review codon changes, GC content, and primer options.

Calculate insert DNA mass for a vector-insert ligation from fragment lengths, vector mass, and the target insert-to-vector molar ratio.

Calculate single-solute mass, molarity, final volume, and g/L; convert protein molarity with kDa; or build multi-solute recipes.

Prepare named buffers by concentration, volume, and target pH, using acid/base calculations or fixed templates for PBS, TBS, TAE, TBE, SSC, and TE.

Calculate C1V1 stock dilutions or antibody working solutions from 1:N ratios or stock-to-target concentrations, with small-volume guidance.